Differential gene expression between wild type and Pax9-null pharyngeal arch tissue

The aim of this study was to identify differentially expressed genes within the pharyngeal arches of Pax9-null embryos at E9.5. Mice null for Pax9 die in the neonatal period with complex cardiovascular defects, caused by abnormal morphogenesis of the pharyngeal arch arteries. Overall design: Total RNA was isolated from dissected pharyngeal arch region of E9.5 mouse embryos. Pax9-null pharyngeal arches were compared to stage-matched wild type controls. Three biological replicates for each group (wild type vs. mutant) were collected. Samples represent a mix of female and male animals. Isolated RNA was subjected to high throughput sequencing analyses to identify differentially expressed transcripts.