Baseline expression from transcriptional profiling of zebrafish developmental stages

Establish baseline expression transcriptional signatures for zebrafish developmental stages. Total RNA was extracted from single wild type zebrafish embryos at a series of different developmental stages. RNA from 12 embryos was pooled to generate a replicate.The RNA was DNase treated. Stranded RNAseq libraries were constructed using the Illumina TruSeq Stranded RNA protocol with oligo dT pulldown.This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/本研究旨在构建斑马鱼不同发育阶段的基线转录表达特征（transcriptional signatures）。从一系列不同发育阶段的单枚野生型斑马鱼胚胎中提取总RNA，将12枚胚胎的RNA混合以制备一个生物学重复样本。所提取的RNA经DNase酶处理。采用Illumina TruSeq链特异性RNA建库方案，结合寡聚dT磁珠富集法构建链特异性RNA测序文库。本数据集属于预发布数据。若需了解威康桑格研究所（Wellcome Trust Sanger Institute）共享的预发布数据的规范使用方式（包括任何出版暂缓的相关细则），请访问http://www.sanger.ac.uk/datasharing/