Fast development of microbial cultures for the anaerobic oxidation of CH4 coupled to denitrification employing widely available inocula

The potential of two inocula found within the wastewater treatment plant to carry out denitrifying anaerobic methane oxidation (N-AOM) employing NO3- as the terminal electron acceptor was tested through batch incubations over a 100-day experiment. The microbial communities found in the inocula as well as during two sampling points during the microbial enrichment process were explored via 16s ILLUMINA sequencing.