CRISPR-based Engineering in Bifidobacterium

NIAID Data Ecosystem2026-03-13 收录

CRISPRi基因编辑

益生菌工程

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA811526 数据链接链接失效反馈

官方服务：

资源简介：

This study developed CRISPR-based genetic engineering in Bifidobacterium

创建时间：

2022-03-01

Use of CRISPR/Cas9 with homology-directed repair to silence the human Topoisomerase IIα intron-19 5' splice site: Generation of etoposide resistance in human leukemia K562 cells. Use of CRISPR/Cas9 with homology-directed repair to silence the human Topoisomerase IIα intron-19 5' splice site: Generation of etoposide resistance in human leukemia K562 cells

CRISPRi基因编辑

治疗抗性

Results support the important role of both TOP2α/170 and TOP2α/90 as determinants of sensitivity/resistance to TOP2α-targeting agents. Overall design: CRISPR/Cas9 with homology-directed repair was uti

NIAID Data Ecosystem40

File S2.zip

CRISPRi基因编辑

基因敲入序列分析

Annotated sequence files in .ape format (comparable with Genbank) of knock-ins generated by CRISPR/Cas9-mediated genome editing. Unmodified genomic sequence is also provided.

Figshare2023-03-14 更新20

EGAS00001006170-sc-2023-10-20T08:25:08Z - samples

DNA提取

CRISPRi基因编辑

8 cell pellet samples for genomic DNA extraction. CRISPR PCR1 and PCR2 indexing - Please use standard Kosuke primers.EGA dataset EGAD00001012233

NIAID Data Ecosystem00

Single-cell data of probiotic engineering

单细胞分析

益生菌工程

This project observed the changes in intestinal cells of colitis mice after probiotic intervention.

DataCite Commons2025-11-22 更新30

sgRNA sequences used for CRISPR targeting of TET1.