Data underlying the publication: Neuroprotective Effects of Everolimus via Regulation of Autophagy-Related Proteins in a Cerebral Ischemia-Reperfusion Rat Model

The data was collected from MCAO rats to evaluate the neuroprotection of everolimus. In excel file, the Longa’s neurological scale was applied to evaluate neurobehavioral deficit in ischemia/reperfusion-induced rats, and the ischemic infarct area of each section was calculated as infarct size. Besides, immunochemical staining of MCAO rats for mTOR and Beclin-1 expression was uploaded to observe the influence of everolimus on mTOR and Beclin-1. Besides, the data also included WB images of pPI3K, PI3K, pAkt, Akt, LC3-I, LC3-II. This study explored the effect of everolimus on the expression of PI3K, LC3, and Akt proteins and further clarified the regulatory mechanism of nerve cell autophagy against neural lesions.

本数据集采集自大脑中动脉闭塞（Middle Cerebral Artery Occlusion, MCAO）大鼠，用于评估依维莫司的神经保护作用。在Excel文件中，采用Longa神经功能评分量表（Longa’s neurological scale）评估缺血再灌注（ischemia/reperfusion）诱导模型大鼠的神经行为缺损，并计算各脑组织切片的缺血梗死面积作为梗死面积参数。此外，本数据集收录了MCAO大鼠哺乳动物雷帕霉素靶蛋白（mammalian Target of Rapamycin, mTOR）与Beclin-1表达的免疫组织化学染色结果，以观察依维莫司对mTOR及Beclin-1的调控影响。同时，数据集还涵盖pPI3K、PI3K、pAkt、Akt、LC3-I、LC3-II的蛋白质印迹法（Western Blot, WB）图像。本研究探讨了依维莫司对PI3K、LC3及Akt蛋白表达的调控作用，并进一步阐明了神经细胞自噬对抗神经损伤的分子调控机制。