Genome Closure of Klebsiella pneumoniae KPC+ Isolates for Variation and Prophage Content Analysis. Klebsiella pneumoniae

These previously sequenced MDR Klebsiella pneumoniae species have, in this project, been additionally sequenced with nanopore technology and assembled into closed single contig genomes with their respective plasmids. These strains are of importance because they are part of a group of high profile pathogens, the carbapenem resistant enterobacteriaceae (CREs). These strains are human isolates and are a part of the medically relevant sequence type ST258, with some including the KPC plasmid encoding for multidrug resistance factors. These complete genomes have allowed for an analysis of prophage content as well as analysis of variation between Klebsiella strains and strain derived mutants relating to bacteriophage research.