Expression of Chondrotin Sulfates on immunopurified CD44 from untreated and PDGF-treated LS174T cells as assessed by flow cytometry.

CD44 expression levels on microspheres were quantified by single-color immunofluorescence and flow cytometry using the PE-conjugated anti-CD44 mAb 515. Expression of chondroitin sulfate chains on CD44-coated microspheres were quantified using the anti-chondroitin-4-sulfate (BE-123) or anti-chondroitin-6-sulfate (MAB2035) antibody or an antibody against chondroitin-4-sulfate stubs ((anti-PG ΔDi-4S) following digestion with Chondroitinase ABC) followed by incubation with a PE-conjugated anti-mouse IgG (H+L) secondary antibody. Data are reported as mean fluorescence intensity and represent the mean±SEM of n = 3 experiments.