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PROPER-seq (PROtein Protein intERaction sequencing)

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE150818
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To reveal protein-protein interactions at transcriptomic scale by sequencing PROPER-seq converts the input transcriptome into a library of RNA-barcoded proteins and subsequently reverse transcribes and ligates the two RNA barcodes of each PPI to yield a chimeric DNA sequence. This approach enables simultaneous conversion of many PPIs into chimeric DNA sequences and subsequent decoding of the PPIs by sequencing these chimeric sequences
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2021-08-31
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