GJB2 c.109G>A mutation causes hereditary non-syndromic deafness through the mitochondrial apoptosis pathway

To further investigate the regulatory pathway of GJB2 c.109G>A mutants in hereditary non-syndromic deafness, we performed a transcriptomic sequencing analysis. Differentially expressed genes between the Ctrl and MO groups were screened based on FDR-p<0.05 and |log2 Fold Change|>0.5. The results showed that 243 differentially expressed genes were screened in the Ctrl and MO groups, with 63 genes downregulated and 180 genes upregulated. These differentially expressed genes were visualized by drawing heatmaps and volcano plots. GO analysis was performed on the differentially expressed genes. GO analysis results indicated that the main enrichment in the Biological Process category was secondary alcohol metabolic process, release of cytochrome c from mitochondria, and defense response to other organism . Overall design: Collected 72 hpf wild-type AB strain zebrafish and GJB2-MO zebrafish for RNA-seq sequencing.