Top 10 off-target sites of the designed sgRNA sequence.

NIAID Data Ecosystem2026-05-01 收录

CRISPR脱靶效应

sgRNA优化

数据链接：

https://figshare.com/articles/dataset/Top_10_off-target_sites_of_the_designed_sgRNA_sequence_/24034570 数据链接链接失效反馈

官方服务：

资源简介：

Top 10 off-target sites of the designed sgRNA sequence.

创建时间：

2023-08-25

Amplicon-based NGS sequencing of CRISPR/Cas9 off-target sites

CRISPR脱靶效应

肠道类器官基因编辑

The APC, KRAS, P53 and SMAD4 genes were mutated in human small intestinal stem cells and colon stem cells, which grow in vitro as epithelial organoids. Here we assessed candidate off-target sites for

NIAID Data Ecosystem30

Rational sgRNA Design for CRISPR/Cas9-mediated Gene Inactivation. Mammalia

sgRNA优化

基因编辑效能预测

Components of clustered regularly interspersed palindromic repeat (CRISPR) loci, which function as an adaptable immune system in many prokaryotes, have recently been repurposed for use in mammalian ce

NIAID Data Ecosystem30

Transcriptional analysis for off-target effects by sgRNA variants in E.coli BL21(DE3)

CRISPR脱靶效应

sgRNA优化

Five different sgRNAs targeting mCherry gene (wild-type, without a spacer, and three truncated variants) were used to investigate the off-target effects of CRISPR interference. Total RNA sequencing da

NIAID Data Ecosystem20

Sequence Requirements for Cascade Function in a Type I-E CRISPR System: RNA-Seq. Sequence Requirements for Cascade Function in a Type I-E CRISPR System: RNA-Seq

CRISPR脱靶效应

基因表达调控

We measured global RNA levels using RNA-seq in Δcas3 E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers aff

NIAID Data Ecosystem30

AlleleAnalyzer supporting data

CRISPRi基因编辑

sgRNA优化

The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distin

DataCite Commons2026-03-11 更新20

Top 10 off-target sites of the designed sgRNA sequence.

资源简介：

相关数据集