CRISPR-Cas9 expression independent of its cognate synthetic guide RNA (gRNA) causes widespread genomic DNA damage in human cells. To investigate whether Cas9 can interact with endogenous human RNA tra

NIAID Data Ecosystem30

Additional file 2 of The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model

CRISPR脱靶效应

差异表达转录组分析

Additional file 2: Table S1. Transcriptome profiles of N2a cells transfected of RfxCas13d and crRNA, and DEGs identified through comparisons (Linked to Figs. 1, 2, and 3 and the answer to the first qu

Figshare2023-02-01 更新20

A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture

CRISPR脱靶效应

基因组编辑特异性

Clustered regularly interspaced short palindromic repeat (CRISPR) RNA-guided nucleases have gathered considerable excitement as a tool for genome engineering. However, questions remain about the speci

NIAID Data Ecosystem40

Analysis of single nucleotide variants in zebrafish embryos after CRISPR-Cas9 editing shows no evidence of off-target inflation

CRISPR脱靶效应

斑马鱼基因编辑安全性

The rapid feasibility of CRISPR-Cas9 gene editing for therapeutic applications has perpetuated the need to assess potentially deleterious off-target editing events. Here we show the importance of cont

NIAID Data Ecosystem20