An alternative pluripotent state confers interspecies chimaeric competency [Microarray]

Here we show that by simple modulation of extrinsic signaling pathways, a new class of pluripotent stem cells, referred to as intrinsic state-epiblast stem cells (IS-EPIs), could be efficiently derived from different stages of the early embryo. IS-EPIs share features of primed pluripotency yet are distinct from EpiSCs in their molecular characteristics and ability to colonize post-implantation embryos. We performed Microarray analysis and compared global gene expression pattern among amplified RNA samples from ESCs, EpiSCS, IS-EPIs as well as in vivo isolated Epiblasts. Examination of global gene expression profiles multiple pluripotent stem cell types本研究证实，通过对外源信号通路进行简单调控，可从早期胚胎不同发育阶段高效分离得到一类新型多能干细胞——本征态上胚层干细胞（intrinsic state-epiblast stem cells, IS-EPIs）。该类细胞具备始发态多能性的特征，但在分子特征及定植植入后胚胎的能力上，与上胚层干细胞（epiblast stem cells, EpiSCs）存在显著差异。本研究开展了基因芯片分析，对胚胎干细胞（embryonic stem cells, ESCs）、上胚层干细胞（EpiSCs）、本征态上胚层干细胞（IS-EPIs）以及体内分离的上胚层的扩增RNA样本的全基因表达模式进行了比较，并对多种多能干细胞类型的全基因表达谱展开了分析。