Kinetics raw dataMolecular and functional disparities of bovine viral diarrhoea virus 1b, 1d, and 1f subgenotype field isolates

Non-cytopathogenic (ncp) and cytopathogenic (cp) pairs of subgenotype 1b and 1f, and a single ncp isolate of the 1d subgenotype isolate of bovine viral diarrhoea virus (BVDV) strains currently circulating in Hungary were submitted to a comparative analysis regarding their in vitro replication kinetics and molecular composition. The kinetic analysis included digital PCR (dPCR) and virus titration. While the dPCR indicated a higher replication ratio for the cp viruses over the course of the 120 hrs observation period, the opposite was seen for all but the 1f ncp isolate by virus titration.The 1d/1b, 1d/1f, and 1b/1f subgenotypes differed in 431-510 amino acids on the polyprotein level. The 1b and 1f ncp/cp counterparts differed only in 9 and 29 amino acids, respectively. Subgenotype specific differences were also revealed in both untranslated regions.A „hallmark” amino acid deletion in the nucleocapsid coding region of the 1d subgenotype was present in the analysed isolate.None of the reported indels responsible for the ncp to cp transversion were identified in the respective pairs, however, amino acid substitutions were found in the Npro, capsid, and NS4B coding regions.Our results demonstrated a subtle but remarkable difference between the detection methods used for kinetic analysis, which can even be more pronounced in vivo, such as evaluating virus shedding results.Further, our findings support the previous observations regarding the complexity of the molecular differences between the biotypes of BVDV.