Identification and characterization of miRNAs in temperature-dependent sex determination (TSD) of Chinese pond turtles (Mauremys reevesii). Mauremys reevesii

In the present study, the eggs of Chinese pond turtles (Mauremys reevesii) were incubated at three temperatures (26℃, 29℃ and 32℃). During the thermosensitive period (TSP) of incubation, the adrenal-kidney-gonad (AKG) complexes were sampled, and a comprehensive investigation for miRNAs was performed using next-generation small RNA sequencing. Overall design: Global miRNA expression kinetics during sex determination in a TSD species have been extensively profiled.