Effect of cigarette smoking on human tracheal epithelium

We used single-cell RNA sequencing (scRNA-seq) to elucidate the effect of cigarette smoking on cell type populations in the human tracheal epithelium Overall design: To investigate the effects of smoking on the human tracheal epithelium, we took tracheal brushings from three never-smoker patients and three current-smoker patients and performed scRNAseq.