miRNA sequencing of synovial fluid derived extracellular vesicles between normal and osteoarthritis (OA) patients

To determine whether extracellular vesicles were also present in human disease, we isolated and evaluated extracellular vesicles from synovial fluid from normal and OA patients at relatively advanced ages (70 - 80 years). Here we report the altered expression of synovial fluid extracellular vesicles-derived miRNAs in osteoarthritis compared to normal patients with age-relate degeneration to investigate potential biomarkers for OA diagnosis. The OA donors had clinical evidence of OA based on pain that led to total joint arthroplasty. Overall design: Examination of miRNA expression in extracellular vesicles isolated from synovial fluids of normal and osteoarthritis patients