Additional file 2 of Engineered virus-like particle-assembled Vegfa-targeting Cas9 ribonucleoprotein treatment alleviates neovascularization in wet age-related macular degeneration
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Additional file 2: Table S1. Oligomers for sgRNA cloning and primers used to amplify the on-target site for NGS. Table S2. Primers used for amplification of off-target sites: Off-targets 1–4 (CCTop-predicted) and Off-targets 5–10 (Digenome-seq-identified with NGG PAM). Table S3. All cleavage sites identified by Digenome-seq and their corresponding DNA cleavage scores.
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figshare
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2025-10-10
