Gene expression analysis of enSLiT KO cells against Salmonella infection

Eukaryotic genomes are pervasively transcribed, resulting in the production of many unstable nuclear long noncoding RNAs such as nuclear Short-Lived noncoding Transcripts (nSLiTs). However, the biological significance and turnover mechanism of nSLiTs are largely unknown. We used microarrays to detail the global program of gene expression that is regulated by enSLiT upon Salmonella infection To assess the role of enSLiT against Salmonella infection, we knockout enSLiT07573 by using CRISPR/Cas9. KO was performed replicate for each transcripts. We comparied Salmonella infected/not infected HeLa cells and KO cells. All data was obtained biological triplicate.