H3K36me3 ChIP-seq of 2 rice lines D248 and SH881

A major maturity duration-regulatory gene, Early flowering-completely dominant (Ef-cd) was cloning in this study. The Ef-cd locus gives rise to a long noncoding RNA (lncRNA) antisense transcript overlapping the OsSOC1 gene. Ef-cd lncRNA expression positively correlates with the expression of OsSOC1 and H3K36me3 deposition. Overall design: Fresh leaf blades of 40 d seedlings of early-maturing NIL D248 (BC5F11) and its recurrent parent SH881 (Shuhui881) were harvested for fixation and immunoprecipitation.