Inflammatory cells and remodeling in bronchial biopsies from COPD patients and controls

The understanding of inflammation and remodeling in the bronchial wall of COPD patients with varying disease severity remains incomplete. 35 healthy controls and 47 volunteer COPD patients underwent bronchoscopy with bronchoalveolar lavage (BAL) and sampling by endobronchial biopsies in 2014-2015 as part of the MicroCOPD Study. Biopsies were embedded in glycol methyl acrylate (GMA) resin and examined by immunohistochemistry and staining for enumeration of CD3+, CD4+, CD8+, CD20+, CD68+, EG2+, and NE+ inflammatory cells, as well as endothelial cells (EN4) and smooth muscle actin (SMA). Mucus cells were stained by periodic acid-schiff (PAS) and toluidine blue to visualize the reticular basement membrane (RBM). The number of macrophages and eosinophils was higher, and vascularity increased in the submucosa in COPD patients compared with healthy controls. In healthy smokers, there were lower numbers of lymphocytes (CD3+, CD4+, CD8+, CD20+) than in never smokers. However, COPD patients with ..., , , # Inflammatory cells and remodeling in bronchial biopsies from COPD patients and controls

#### Description of the data and file structure

Observational bronchoscopy study of COPD patients and Controls with bronchoalveolar lavage from the middle lobe and bronchial biopsies from the right lower lobe. Biopsies underwent GMA embedding and were subsequently stained for immunohistochemistry.

#### Files and variables

**File: ToDRYAD_Oct25_2.dta**

**Description:** 

Stata (.dta) data file \"ToDRYAD_Oct25_2.dta\"

Stata ([www.stata.com](http://www.stata.com)), which is a commercial software company, and use of its program require licensing.

**File: ToDRYAD_Oct25_2.xls**

**Description:** An export of the Stata file \"ToDRYAD_Oct25_2.dta\" (lack label values defined since excel does not have that), which can be opened by R or other readily available programs.

Variables within the \"ToDRYAD_Oct25_2.dta\" and \"ToDRYAD_Oct25_2.xls\" files:

* category - controls or COPD

- agecat - age in three cat..., Participants permitted the publication of anonymized results from the study. All data is anonymized (the study ID number is not included, and one cannot back-track from the current data file to the original study participants. Only the metadata used for the publication in PLOS One is provided here, and only on a sub-sample of all study participants in the MicroCOPD study. Age has been binned into categories for an extra layer of anonymity.