Triggering of Endoplasmic Reticulum Stress via ATF4-SPHK1 Signaling Promotes Glioblastoma Invasion and Chemoresistance

After treated with temozolomide for 3 day, LN229 cells were crosslinked and centrifugation. Then it was immunoprecipitated with ATF4 antibodies. DNA was pulled down with Protein A+G beads and purified from DNA-protein complex for sequencing. After CHIP DNA was purified, the conduction of sequencing was on Illumina HiSeq platform by Personal Biotechnology company (Shanghai, China). Reads were mapping to human (Homo sapiens. GRCh38) genome and carried out by Bowtie2 software, and unique mapping reads were selected for the downstream analysis.