RNA profiling of genistein and Tanshinone I treated cervical cancer cells

Phenotypically, we observed that both genistein and Tanshinone I exert the inhibitory effects on the proliferation and metastasis of cervical cancer. The aim of this study was to comprehensively decipher the anti-metastasis effect and molecular mechanism of genistein and Tanshinone I on the cervical cancer. The results showed that genistein and Tanshinone I strongly change the RNA profiling of the cervical cancer cells in different manners: genistein mainly targets on regulation of RNA transcription, central carbon metabolism in cancer, microRNAs in cancer and focal adhesion etc; Tanshinone I mainly effects on central carbon metabolism in cancer, mitophagy process. In this study, we aim to elucidate the changes of mRNA profiles of human cervical cancer due to genistein and Tanshinone I treatments. We designed our experiments in three groups: Hela cells from human cervical cancer were treated with I) 50 μM genistein, labeled as Geni; ii) 50 μM Tanshinone I, labeled as TanI; and iii) 50 μM solvent control, labeled as Ctrl for 5h. Three replicates were set for each individual group.