Heterogeneity in allospecific T cell function in transplant tolerant hosts determines susceptibility to rejection following infection

CD4+CD45.1+ TCR75/Rag-/- T cells (TCR75) were adoptively transferred into CD45.2+ B6 hosts either untransplanted and immunized with B/c donor splenocytes i.p. (UnTx+DST) to induce memory, or prior to transplantation of a B/c heart (HTx) in the presence of anti-CD154 (day 0, day 7, day 14) and DST (day 0) to induce tolerance (Tol). A group of tolerant mice were infected with Lm on d30 post-transplantation (Tol+Lm). CD45.1+ TCR75 cells were harvested from the spleen and lymph nodes on d35-37 post-transplantation in all groups, enumerated and subjected to bulk RNAseq.