GNPS - Carnitine analyses of hylid tree frogs (Amphibia: Anura)

Samples were obtained from the dorsal skin of frogs after a mild electrical stimuli. They were freeze-dried and resuspended in water: acetonitrile (7:3) with 0.1% (v/v) trifluoroacetic acid (TFA). The aim of the analyses was to identified secondary metabolites in particular from the Boana pulchella group. After isolation and characterization of two novel macrocyclic acylcarnitines (MACH) by a combination of LC-MS/MS, RMN and GC/MS we also putatively identified other substances from the same series.