Hydrodynamic dimensions of WT and mutant pVHL proteins.

Size exclusion was performed in high salt buffer in the presence and absence of Arginine. Apparent molecular mass of WT and mutant pVHL with (left) and without Arginine (right) was calculated with the curve equation Kav versus Log Mw. Apparent Rs of WT and mutant pVHL with (left) and without Arginine (right) was calculated with the curve equation √-logKav versus Rs(nm).