A Genome-Wide CRISPR Screen Identifies BRD4 as a Regulator of Cardiomyocyte Differentiation [in-vivo scRNA-seq]

We employed single cell RNA sequencing to interrogate the consequence of conditional Brd4 deletion in the anterior heart field on heart development. Examination of single cell transcriptomes from cardiac progenitors captured at E9.5 from Brd4 floxed homozygous and Brd4 floxed het; Mef2c-AHF-cre hets. At least 2 biological replicate embryos were analyzed per genotype.本研究采用单细胞RNA测序（single cell RNA sequencing），探究前心区（anterior heart field）内条件性Brd4基因敲除对心脏发育的影响。我们对两种基因型小鼠胚胎在E9.5时期分离得到的心脏祖细胞的单细胞转录组进行了检测分析：一组为Brd4 floxed纯合子（Brd4 floxed homozygous），另一组为Brd4 floxed杂合且Mef2c-AHF-cre杂合的个体；每种基因型均至少分析2个生物学重复胚胎。