Research data supporting "High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data"

This directory contains all the original data from the publication
"High-throughput 3D phenotyping of plant shoot apical meristems from
tissue-resolution data".

Title: Research data supporting High-throughput 3D phenotyping of plant shoot apical meristems from tissue-resolution data
Authors: Henrik Åhl, Yi Zhang, Henrik Jönsson
Contact: [e-mail]
Date of data collection: 2017-2021
Associated DOI: 10.3389/fpls.2022.827147
Location: Sainsbury Laboratory, University of Cambridge
Keywords: pin3, pin4, pin7, pin3pin4, pin3pin7, pin4pin7, pin3pin4pin7, aux1, aux1lax1lax2lax3, abcb1, abcb19, abcb1abcb19, pCLV3:dsRed x pPIN1:PIN1-GFP x myr-YFP, xxt1xxt2, xxt1xxt2xxt5, qua1, bag, cesa3(eli1), cesa6(prc1-1), csi1-3, det3, cesa1(any1), gabi, cu4, cmu1cmu2, cesa3(je5), act2-5, arabidopsis thaliana, shoot apical meristem, confocal, isosurface, phyllotaxis, apex identification, tissue segmentation, plant development, flower development, 3D phenotyping, high-throughput
Microscopes: LSM880, LSM700, LSM780
Formats: .csv, .txt, .lsm, .tif

This dataset contains 3D image stacks of the following:

- Col-0 plant shoots
- pin3, pin4, pin7, pin3,4, pin3,7, pin4,7, pin3,4,7 mutant plants
- aux1, aux1lax1,2,3 mutant plant shoots
- abcb1, abcb19, abcb1,19 mutant plant shoots
- pCLV3::dsRed x pPIN1:PIN1-GFP x myr-YFP expressing plant shoots
- xxt1xxt2, xxt1xxt2xxt5, qua1*, bag*, cesa3(eli1), cesa6(prc1-1), csi1-3*, det3*, cesa1(any1), gabi*, cu4*, cmu1cmu2*, cesa3(je5), act2-5* mutant plant shoots (*: not used in the analysis)
- One pABCB:ABCB19-GFP expressing plant
- One Col-0 protoplast
- One Col-0 apical hook

The dataset also contains the output results from the analysis pipeline presented in the affiliated publication, as well as corresponding file metadata.

Code and scripts used for data generation and statistical analysis, including visualisation can be found at https://gitlab.com/slcu/teamHJ/publications/aahl_etal_2022.

本目录收录了发表于《从组织分辨率数据中进行高通量3D表型分析植物茎尖分生组织》一文的原始数据。

标题：支持《从组织分辨率数据中进行高通量3D表型分析植物茎尖分生组织》的研究数据

作者：Henrik Åhl, Yi Zhang, Henrik Jönsson

联系方式：[e-mail]

数据收集日期：2017-2021

关联DOI：10.3389/fpls.2022.827147

地点：剑桥大学Sainsbury实验室

关键词：pin3（植物细胞极性蛋白）、pin4、pin7、pin3pin4、pin3pin7、pin4pin7、pin3pin4pin7、aux1、aux1lax1lax2lax3、abcb1、abcb19、abcb1abcb19、pCLV3:dsRed x pPIN1:PIN1-GFP x myr-YFP、xxt1xxt2、xxt1xxt2xxt5、qua1、bag、cesa3(eli1)、cesa6(prc1-1)、csi1-3、det3、cesa1(any1)、gabi、cu4、cmu1cmu2、cesa3(je5)、act2-5、拟南芥（Arabidopsis thaliana）、茎尖分生组织、共聚焦显微镜、等值面、叶序、顶点识别、组织分割、植物发育、花发育、3D表型分析、高通量

显微镜：LSM880、LSM700、LSM780

格式：.csv、.txt、.lsm、.tif

本数据集包含以下3D图像序列：

- Col-0植物茎尖

- pin3、pin4、pin7、pin3,4、pin3,7、pin4,7、pin3,4,7突变体植物

- aux1、aux1lax1、2、3突变体植物茎尖

- abcb1、abcb19、abcb1,19突变体植物茎尖

- 表达pCLV3::dsRed x pPIN1:PIN1-GFP x myr-YFP的植物茎尖

- xxt1xxt2、xxt1xxt2xxt5、qua1*、bag*、cesa3(eli1)、cesa6(prc1-1)、csi1-3*、det3*、cesa1(any1)、gabi*、cu4*、cmu1cmu2*、cesa3(je5)、act2-5*突变体植物茎尖（*：未用于分析）

- 一株表达pABCB:ABCB19-GFP的植物

- 一株Col-0原生质体

- 一株Col-0茎尖钩

此外，本数据集还包含相关附属出版物中提出的分析流程的输出结果以及相应的文件元数据。

用于数据生成、统计分析（包括可视化）的代码和脚本可从以下链接获取：https://gitlab.com/slcu/teamHJ/publications/aahl_etal_2022。