Effect of nAChRbp on the ability J2 Globodera pallida to respond chemotactically to root exudate.

Nematodes were exposed to three concentrations of the peptide or to root exudate from transgenic potato plants secreting the peptide. Their subsequent movement on agar plates into a central zone surrounding non-transgenic potato root exudate was monitored over a 3 h period. Nematodes were scored into three groups from the tracks on agar relative to root exudate placed at the centre of the Petri dish. Some moved repeatedly to within 0.5 cm of the dried exudate, others did so once while many never approached the centre of the plate. The χ2 values were calculated after correction for continuity.