N-glycosylation sites of Phlebotomus tobbi salivary proteins.

Selected mature proteins from Phlebotomus tobbi were subjected to the NetNGlyc 1.0 Server [48] using the default setting (by default, predictions are done only on the Asn-Xaa-Ser/Thr sequons and the treshold is set up at 0.5). Cluster names and GenBank accession numbers are indicated. Molecular weight (kDa) was calculated based on the Figure 1A. The Position column defines predicted glycosylated sites. The Potential score is the averaged output of nine neural network, and the Jury agreement column indicates how many of the nine networks support the prediction. The N-Glyc Result column shows putative glycosylated sites: + denotes Potential >0.5, ++ Potential >0.5 and Jury agreement (9/9) or Potential >0.75, +++ Potential >0.75 and Jury agreement, ++++ Potential >0.90 and Jury agreement.*Proline occurs just after the asparagine residue that makes it highly unlikely that the asparagine is glycosylated, presumably due to conformational constraints. ConA column shows reactivity with lectin concanavalin A based on the Figure 22, lane 4.