Optimization of the Assay for Primers and Probes.
收藏NIAID Data Ecosystem2026-03-07 收录
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The Quantitative Real Time PCR assay was first performed with varying concentrations of primer and then probe to determine the optimal concentration of primers and probe for the assay. The concentration at which, for both primers and probe, the ΔCt and ΔRn did not change with increasing concentrations was selected. Thus primer concentrations of 600 nM and probe concentration of 250 nM were chosen.
创建时间：
2013-02-21
