Analysis of the Saccharomyces cerevisiae Pan-Genome Reveals a Pool of Copy Number Variants Distributed in Diverse Yeast Strains From Differing Industrial Environments.

We created a multi-species microarray platform, containing probes to the whole genomes of seven different Saccharomyces species, with very dense coverage (one probe every ~500 bp) of the S. cerevisiae genome, including non-S288c regions, mitochondrial and 2 micron circle genomes, plus probes at fairly dense coverage (one probe every ~2,100 bp) for each of the genomes of six other Saccharomyces species: S. paradoxus, S. mikatae, S. kudriavzevii, S. bayanus, S. kluyveri and S. castellii本研究构建了一款多物种微阵列（microarray）平台，该平台包含针对7种不同酿酒酵母属（Saccharomyces）物种全基因组的探针。其中针对酿酒酵母（Saccharomyces cerevisiae）基因组的覆盖度极高，每约500 bp设置1条探针，覆盖范围涵盖非S288c区域、线粒体基因组及2微米环状基因组；此外还为其余6种酿酒酵母属物种的基因组提供了密度较为可观的探针覆盖，每约2100 bp设置1条探针，涉及物种依次为S. paradoxus、S. mikatae、S. kudriavzevii、S. bayanus、S. kluyveri及S. castellii。