Gene expression data from human breast cancer MCF-7 cells and its 4-hydroxytamoxifen-resistant derivative OHTR cells treated with an siRNA targeting TRIM47

To evaluate the effect of TRIM47 on NF-κB signaling in breast cancer cells, expression of TRIM47 was knocked down in MCF-7 cells and its 4-hydroxytamoxifen (OHT)-resistant derivative OHTR cells by small interfering RNA (siRNA). Microarray analysis was performed to analyze gene expression profiles of these cells. MCF-7 and OHTR cells were treated with a specific siRNA targeting TRIM47 and a control siRNA without specific targets. Total RNA was extracted from the cells for gene profiling 48 h after transfection of siRNA. RNA integrity number (RIN) was measured by Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). It was confirmed that RINs of all RNAs were above 9. RNAs extracted from biological triplicates treated with the same siRNA were combined, and subjected to the microarray analysis using Clariom S Array, Human (Affymetrix/Thermo Fisher Scientific, Walthan, MA, USA).