Moderate nutritional stress reprograms insulin responses to drive enhanced starvation tolerance in Drosophila melanogaster

The datasets contain information regarding the effects of brief exposure to starvation stress on the physiology and metabolism of Drosophila melanogaster.The flies that have undergone previous exposure to starvation stress (trained flies) show increased resistance to starvation in comparison to the fed counterparts. They also show a differential utilization of energy reserves namely triglyceride, glycogen, glucose, and trehalose in response to starvation.Further, we show that the insulin responses of these flies are different. Trained flies maintain high insulin signaling during starvation and are insulin-sensitive.Briefly, through various assays such as starvation assay, metabolic assay, stress assays, western blots, quantitative real time polymerase chain reaction (qRTPCR), immunohistochemistry, etc. we show that prior exposure to starvation stress rewires physiology and metabolism of trained flies.The data has been compiled into one file. The images are added to a separate folder.