Analysis of parasite clones from different transfections to confirm proper integration into the genome by PCR and qPCR.

Plasmids were either transfected in circular form (cir) or were linearized with BglI before transfection (lin). Shown are all the clones analyzed for every transfection that was recovered. Integration was confirmed by PCR of the 5’ and 3’ region and appearance of a band at the right size was considered a positive result (green check mark). The absence of the transfection plasmid was screened for with primers targeting the backbone of the plasmid which is lost after correct integration (red cross). Quantitative PCR was also used to confirm copy numbers of plasmepsin II and III as well as the hdhfr gene inserted into the locus. Primers are listed in S11 Table.
(XLSX)