rat gut metagenome Raw sequence reads

High-performance liquid chromatography (HPLC) was used to identify the components of pea oligosaccharide (PGOS) by comparing retention time of reference substance. The beneficial effect of PGOS ingestion on intestinal health was investigated, including the ability to regulate the intestinal microbiota composition using 16S rRNA gene sequences and short chain fatty acids (SCFA) contents using targeted metabonomic analysis. And then, the relationship between the dominant genus and the production of SCFA was established. Finally, other possible functions of microbial genus that may be beneficial to host intestinal health were predicted. Results suggest that PGOS are composed of sucrose, D-melibiose monohydrate, D-raffinose pentahydrate, manninotriose, stachyose hydrate and verbascose, which account for 61.56% of the total components. PGOS significantly increased the content of acetic acid, propionic acid, butyric acid, valerate acid and hexanoic acid in feces, even better than galactooligosaccharides (GOS). Both PGOS and GOS regulate the composition and relative abundance of intestinal microflora of rats. In terms of beneficial bacteria, GOS and PGOS promote the proliferation of Lactobacillus and Prevotella in the gut. In addition, unclassified f_ prevotellaceae, Alloprevotella, Prevotellaceae UCG-001 and Prevotellaceae NK3B31 group were all homologous with Prevotella, and increased in rat intestines, all of which are positively correlated with the measured SCFA. Judging from the harmful bacteria, PGOS could reduce the abundance of Dubosiella, Allobaculum, and Faccalibaculum that were negatively correlated with SCFA production, and the latter two originated from Dubosiella. Moreover, PGOS reduced the abundance of Blautia, Bacteroids, Lachnoclostridium and Turicibacter, negatively correlated with SCFA, while GOS increased the abundance of most of these genera.