Phagocytosis index and rate, percentage of binding and number of apoptotic and autophagic HeLa cells bound per isolated human macrophages.

Phagocytosis index: number of dead cells internalized per macrophage.
Phagocytosis rate: number of macrophages internalizing at least one dead cell.
Percentage of binding: number of macrophages binding at least one dead cell.
Viable: untreated HeLa cells; RBAc-PDT (12 h rec): apoptotic PDT-induced in the presence of 3-MA (10 mM) and Nec-1 (300 µ) HeLa cells, 12 h of recovery post PDT; RBAc-PDT (8 h rec): autophagic PDT-induced in the presence of z-VAD-FMK (20 µ) and 3-MA (10 mM) HeLa cells, 8 h of recovery post PDT.
N.D. Not Detected value, corresponding to 0 cells counted.
RBAc-PDT: HeLa cells incubated with Rose Bengal Acetate for 60 min followed by 90 seconds irradiation with green light (1.6 J/cm2) and then by recovery in fresh medium (8 and 12 h).
Each value is the average ±SD of 500 cells scored out of three independent experiments. Asterisks show significant (-anti-CRT) values (p<0.05) versus (+anti-CRT) ones.