Selection for late reproduction leads to loss of complex I mitochondrial capacity and associated increased longevity in seed beetles

Mitochondria play a key role in aging. Here, we measured integrated mitochondrial functions in experimentally evolved lines of the seed beetle Acanthoscelides obtectus that were selected for early (E) or late (L) reproduction for nearly four decades. The two lines have markedly different lifespans (8 days and 13 days in the E and L lines, respectively). The contribution of the NADH pathway to maximal flux was lower in the L compared to the E beetles at young stages, associated to increased control by complex I. In contrast, the contribution of the Succinate pathway was higher in the L than in the E line, while the Proline pathway showed no differences between the lines. Our data suggest that selection of age at reproduction leads to a modulation of complex I activity in mitochondria and that mitochondria are a functional link between evolutionary and mechanistic theories of aging., See methods in the paper., , # Selection for late reproduction leads to loss of complex I mitochondrial capacity and associated increased longevity in seed beetles

## **Description of the Data and file structure**

Date of data collection are indicated in the title of each set in the column A.

Information about geographic location of data collection: Edmonton, Alberta, Canada.

Keywords used to describe the data topic: included in the datasheet.

Language information: English.

Method used to collect the data: High resolution respirometry (O2k, Oroboros Instrument, Innsbruck, Austria) and datlab software 7 (Oroboros Instrument).

The data in red were in chamber A of an oxygraph, and the data in green were from chamber B of an oxygraph.

The abbreviations used in each of the data sheet are the following:

E: Early line

L: Late line

FEM: Females

PMG-L: Pyruve+Malate+Glutamate, No ADP

PMG-D: Pyruve+Malate+Glutamate+ADP

PMG-Dc: Pyruve+Malate+Glutamate+ADP+cytochrome c

PMGS-Dc: Pyruve+Malate+Glutamate+Succinate+...