Data underlying the chapter Discovery and synthetic applications of thermostable NAD(P)H-dependent reductive aminases

This dataset contains data collected during experiments at Delft University of Technology, as a chapter of the dissertation written by Ewald Jongkind .The aim of this work was to characterize Reductive Aminases (RedAms) from databases from our collaborators. This was done by expressing potential RedAm genes from a gene database and characterize the RedAms. Dataset includes protein purification gels images, calculated enzyme concentrations, calculated conversions by the enzymes and enzymatic activities in Excel. More details are found in the publication and the README-file.

本数据集包含代尔夫特理工大学（Delft University of Technology）实验中采集的数据，系Ewald Jongkind所撰博士学位论文的一章内容。本研究的核心目标为对合作者提供的数据库中的还原氨基化酶（Reductive Aminases, RedAms）开展特性表征工作。该研究通过从基因数据库中调取潜在的RedAm基因进行表达，随后对该类酶完成特性表征得以实现。本数据集涵盖Excel格式存储的蛋白质纯化凝胶图像、酶浓度计算值、酶催化转化率计算值以及酶活性数据。更多详细信息可参阅相关发表文献及README文件。