High-throughput Sequencing Reveals Distinct Location Dependent Changes in the Ileal and Fecal Microbiomes of Nursery Pigs Liquid Fed Fermented DDGS. pig gut metagenome

The objective of the study was to determine the effects of including partially fermented DDGS in liquid diets fed to weanling pigs on the establishment of the intestinal microbiome. DDGS was fed in a liquid diet (25% dry matter (DM)), to pigs with β-glucanase, xylanase and silage inoculant (Pediococcus pentosacaeus 12455 and Propionibacterium jensenii 30081/g DDGS). DDGS was either fermented 1 to 7 days with additives prior to feeding (16% DM, FER DDGS) or added to feed as dry DDGS with additives at the time of liquid feeding (UNFER DDGS). Piglets used in the experiment were purebred Yorkshire and crossbred (Yorkshire*Landrace). Prior to weaning, pigs were ad libitum fed a commercial creep feed (Floradale Feed Mill, Floradale, ON, Canada). At 20 days of age pigs were weaned and randomly assigned to dietary treatments; castrated male and female piglets, as well as littermates, were equally distributed across treatments. On d 42 post-weaning ileal mucosa and digesta, and fecal samples were obtained for bacterial 16S rRNA gene sequencing using MiSeq Illumina platform. Feeding FER DDGS reduced alpha-diversity in the ileal mucosa and feces.