Bulk RNA-seq of in vitro-expanded human spinal cord central canal-derived cell cultures

Adult human spinal cord tissue was obtained from organ donors under institutional research ethics approval. Primary spinal cord central canal-derived cell cultures were established and expanded in vitro. Bulk RNA sequencing was performed on six donor-derived cultures. Total RNA was extracted and libraries were prepared using the Illumina TruSeq Stranded mRNA kit. Sequencing was performed on an Illumina NovaSeq 6000 (paired-end, 150 bp). Transcript abundances were quantified using Salmon against the GENCODE v35 transcriptome. Raw FASTQ files and processed quantifications are provided. Overall design: Bulk RNA sequencing was performed on six independent donor-derived human spinal cord cell cultures. Each sample represents a single donor-derived culture and constitutes a biological replicate. No dye-swaps were performed. The dataset does not include experimental treatment groups or perturbations. All samples were processed using the same library preparation and sequencing platform.