Natural scrapie isolates transmission results to TgOvPrP4 ovine transgenic mice.

(1)Animals were inoculated intracerebrally with 2 mg brain tissue.(2)Genotype of sheep or goat (amino acids 136, 141, 154, and 171).(Goat)Goat isolate.(α)Isolates detected in the flock α.(β)Isolates detected in the flock β.Natural inoculums were divided into 4 different groups, all originating from active surveillance of TSEs in small ruminants. Group 1a: atypical scrapie cases with prnp alleles associated with increased susceptibility to atypical scrapie (AF141RQ or AHQ). Group 1b: atypical scrapie cases without prnp alleles associated with increased susceptibility to atypical scrapie. Group 2: classical usual scrapie cases. Group 3: CH1641-like scrapie cases. Detection of PrPres/PrPsc was performed by Western blot or IHC analyses.