TNF plays a crucial role in inflammation by signaling via T cell TNFR2 [bulkRNA-seq]

TNF regulates the inflammatory cytokine secretion from CD4+ T cells in vitro. Overall design: In vitro experiment (Bulk seq): Naïve CD4+ T cells from WT or Tnfr2-/- mice were isolated from spleen and stimulated with anti-CD3/CD28 for 3 days in the presence of the different combinations of cytokines (WT: TNF+TGF-ß, Tnfr2-/-: TNF+TGF-ß; WT: IL-6+TGF-ß) followed by bulk RNA sequence. Each experimental condition was done triplicates and analyzed.