Teasing apart the many effects of lighting environment on opsin expression and foraging preference in bluefin killifish

Coloration and color vision covary with lighting in many taxa. Determining the mechanisms underlying these patterns is difficult because lighting environments can have multiple effects on signaling that occur at multiple time scales. Lighting environments can (1) immediately affect signal propagation and transmission which determine the radiance spectrum reaching the receiver, (2) induce variation in visual systems via developmental plasticity, and (3) lead to genetic differences in visual systems due to a history of selection in different habitats. We tease apart these effects on pecking preference and examine the relationship between pecking preference and opsin expression. Using killifish from two visually distinct populations (clear vs. tea-stained water), we performed crosses (genetics), raised animals under different lighting conditions (developmental plasticity), and assayed preference to peck at different colored dots under different lighting conditions (immediate effects). P...