RNA Extraction to Protein Expression Analysis by SDS-PAGE
收藏官方服务：
资源简介：
A comprehensive molecular biology lab report detailing total RNA extraction via the TRIzol method, denaturing agarose gel electrophoresis, cDNA synthesis by RT-PCR, directional primer design, PCR amplification, gel extraction, cloning into pJET1.2 and pET-28a(+) vectors, chemical competence preparation ($CaCl_2$ method), heat-shock transformation, colony PCR screening, plasmid miniprep extraction, restriction digestion analysis, IPTG induction, and recombinant protein expression analysis via SDS-PAGE.
提供机构：
Zenodo创建时间：
2026-09-16
