Effect of sulfur starvation on the transcription of sulfur metabolism genes of A. flavipes.

Sulfur starved and non-starved spores of A. flavipes were transferred to basal Dox’s medium containing MgSO4, L-methionine, cysteine, and cystine. Total RNA from the fungal cultures of these treatments was reverse transcribed and used as template in Reverse Transcription (RT)-qPCR using gene-specific primers for the indicated enzymes. Transcription levels were normalized to the transcription of constitutively expressed Actin A gene. Fold changes in transcription of starved cultures were calculated relative to non-starved controls.