Distinct myeloid progenitor differentiation pathways uncovered through single cell RNA sequencing

Haematopoietic stem cells can differentiate into all blood cell types. In this process, cells become progressively restricted to a single cell type. The order in which differentiating cells loose lineage potential, and the prospective isolation of cells with a defined potential remains a long-standing question. We performed gene expression analysis of haematopoietic cells from Gata1-EGFP reporter mice, leading to a model for hematopoiesis where the initial lineage decision consists of a seperation of erythroid/megakaryocyte/mast cell/eosinophil potential from lymphopoietic/monocyte/neutrophil potential Find unbiased heterogeneity in the preGM hematopoietic progenitor population造血干细胞（Haematopoietic stem cells）可分化为所有血细胞类型。在此过程中，细胞会逐步受到谱系限制，最终仅能分化为单一细胞类型。分化细胞丧失谱系潜能的时序规律，以及分离获得具有明确分化潜能细胞的方法，始终是长期悬而未决的学术问题。 我们对来自Gata1-EGFP报告基因小鼠的造血细胞开展了基因表达分析，由此构建了造血生成模型：该模型中，初始谱系决策环节将红细胞/巨核细胞/肥大细胞/嗜酸性粒细胞谱系潜能，与淋巴细胞/单核细胞/中性粒细胞谱系潜能相互分离。本研究还在preGM造血祖细胞群（preGM hematopoietic progenitor population）中发现了无偏倚的细胞异质性。