Next Generation Sequencing of Wild Type and Mice Knocked Out for Known Methionine Sulfoxide Reducatases

Methionine sulfoxide reductase (Msr) is an important antioxidant enzyme. The mammalian Msr family has four members: MsrA, MsrB1, MsrB2, and MsrB3. We generated a mouse strain with deletion of all four Msr proteins. Deletion was confirmed by Western blotting. The quadruple knockout mouse is viable, and growth, development, and fertility appear normal. However, they are unexpectedly resistanct to oxidative stresses. We therefore determined the RNA expression profile in liver and heart of wild-type and quadruple knockout mice. Cardiac and liver mRNA profiles of 3-6 month old wild type and quadruple knockout female mice were generated by deep sequencing, using Illumina Hiseq3000.甲硫氨酸亚砜还原酶（Methionine sulfoxide reductase, Msr）是一类重要的抗氧化酶。哺乳动物的Msr家族共包含四个成员：MsrA、MsrB1、MsrB2与MsrB3。我们构建了同时敲除全部四种Msr蛋白的小鼠品系，并通过蛋白质印迹（Western blotting）验证了基因敲除的有效性。该四基因敲除小鼠可正常存活，其生长、发育及生育能力均表现正常。然而，它们却意外地对氧化应激具有抗性。为此，我们对野生型与四基因敲除小鼠的肝脏和心脏组织开展了RNA表达谱分析。我们采用Illumina Hiseq3000测序平台，通过深度测序获取了3~6月龄野生型及四基因敲除雌性小鼠的心脏与肝脏mRNA表达谱数据。