Next Generation Sequencing Facilitates Quantitative Analysis of wild type and Lmna R527C mutant mice spleen Transcriptomes with or without 4Gy irradiation.

To induced the atypical progeria symptoms of LMNA R527C mutant mice, mutant and wild type mice were irradiated at 6 weeks of age with doses of 4 Gy. And to examine the pathologic change of mutant mice, mice were sacrificed at day -1and day 7 post-irradiation and spleen was harvested for RNAseq. To elucidate the mechanism by which LMNA R527C mutant mice caused senescence, we performed whole genome wide RNA-seq of mutant and wild type mice with or without 4Gy irradiation, and compared the differentially expressed genes between LMNA mutant and wild type mice.