Prasanna Satpute-Krishnan (2010) CIL:9973, Rattus, permanent cell line cell, permanent cell line cell, Kidney. CIL. Dataset

Normal Rat Kidney (NRK) cells grown in culture expressing Galactosyl Transferase-YFP (GalT-YFP) and p58-CFP. This file is the YFP time series demonstrating the organization of the early secretory pathway (Golgi and intermediate compartment).

Images were collected on a Zeiss Spinning Disk confocal as a single z slice over time with CFP imaged using 445ht and YFP imaged using 515ht and a 63X oil objective. Images were collected every 2 minutes. Exposure time: 515 was 50ms; 445 was 200ms: for both channels, the gain was 3, the camera intensification was 200, the laser neutral density was 10.

在培养条件下生长的正常大鼠肾脏（NRK）细胞，表达半乳糖基转移酶-YFP（GalT-YFP）和p58-CFP。本文件展示了早期分泌途径（高尔基体和中间隔室）的组织结构的时间序列YFP图像。图像在Zeiss转盘式共聚焦显微镜下，以单层z切片的形式随时间采集，使用CFP的445ht波长和YFP的515ht波长进行成像，并采用63倍油镜。图像每2分钟采集一次。曝光时间：515波长的曝光时间为50毫秒；445波长的曝光时间为200毫秒；对于两个通道，增益均为3，相机增强度为200，激光中性密度为10。