Dose-dependent gene expression analysis of cardiomyocytes treated with DEHP (1-50 ug/mL)

We used microarrays to expression profile cardiomyocytes from neonatal Sprague-Dawley rats treated with 1 to 50 ug/mL DEHP and control (0.1% DMSO) to identify changes in gene expression related to connexin-43 expression, calcium handling, arrhythmogenesis and mechanical motion.我们采用基因芯片（microarrays）技术，对经1~50 μg/mL邻苯二甲酸二(2-乙基己)酯（DEHP）处理的新生斯普拉-道来（Sprague-Dawley, SD）大鼠心肌细胞，以及以0.1%二甲基亚砜（DMSO）作为对照的细胞样本开展表达谱分析，以鉴定与连接蛋白43（connexin-43）表达、钙代谢、心律失常发生及机械活动相关的基因表达变化。