CHEMICAL COMPOSITION, ANTIRADICALAR AND ANTIMICROBIAL ACTIVITY OF FABACEAE POLLEN BEE

The aim of this investigation was to analyze two samples of pollen collected by Apis mellifera bees in view of their chemical composition, antiradicalar and antimicrobial activities. Palynological analysis showed that the predominant pollen was Fabaceae species (42.3%, sample I and 50.6%, sample II). The characterization by ultra-performance liquid chromatography coupled with a diode array detector and quadrupole time of flight mass spectrometry (UPLC-DAD-QTOF-MS/MS) resulted in the identification of three derivatives of putrescine (di-4-coumaroylputrescine, coumaroyl-feruloyl-putrescine and di-feruloyl-putrescine), two spermidine derivatives (coumaroyl-di-cafeoyl-spermidine and tri-coumaroyl-spermidine and four biflavonoids (2,3-di-hydrohydroxy-amentoflavone, 2,3-dihydro-amentoflavone, amentoflavone and 2,3-dihydro-biapigenin). The two samples showed antiradical and antimicrobial activities, probably due to the presence of these compounds.